GLDC CLIA Kit

Catalog No. ABIN5654738

Human GLDC ELISA Kit Chemiluminescent assay for quantification of Human GLDC.

Quick Overview for GLDC CLIA Kit (ABIN5654738)

Target: GLDC (Glycine Dehydrogenase (GLDC))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details GLDC CLIA Kit

Minimum Detection Limit: 156.25 pg/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glycine Dehydrogenase (GLDC). No significant cross-reactivity or interference between Glycine Dehydrogenase (GLDC) and analogues was observed.

Sensitivity: 4.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glycine Dehydrogenase (GLDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glycine Dehydrogenase (GLDC) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: GLDC (Glycine Dehydrogenase (GLDC))

Alternative Name: Glycine Dehydrogenase

Background:

Gene Name: Glycine Dehydrogenase

Gene Aliases: GCSP, NKH, Decarboxylating, Glycine Decarboxylase, Glycine Cleavage System Protein P, Glycine dehydrogenase (aminomethyl-transferring)

Gene ID: 2731

UniProt: P23378